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My Research

My contributions in science have focused on the realm of a technology called Nanopore DNA sequencing. I like to say that this technology is akin to a 'DNA-scanner.' Because DNA is really small, about 100,000 times smaller in diameter than a human hair. The scanner needs to be really small, hence the 'nano'-pore!

My efforts led the way for the first functional technology with several fundamental contributions. My contributions continue in the industry as I coordinate teams and help develop the Machine Learning that enables the technology's full potential.

Below links to my 'bio info story' describing how genetic information flows with next-gen sequencers and my academic publications. If you have questions, don't hesitate to get in touch. 

Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage

M.T. Noakes, H. Brinkerhoff, A.H. Laszlo, I.M. Derrington, K.W. Langford, J.W. Mount, J.L. Bowman,
K.M. Doering, B.I.Tickman, J.H. Gundlach. Nature Biotechnology doi: 10.1038/s41587-019-0096-0

Nature Biotechnology

Herein we use DNA's inherent flexibility to create a more 'continuous scan' of the DNA as it moves through the nanopore to increase sequencing accuracy.

Subangstrom single-molecule measurements of motor proteins using a nanopore.

I.M. Derrington, J.M. Craig, E.Stava, A.H. Laszlo, B.C. Ross, H. Brinkerhoff, I.C. Nova, K. Doering, B. Tickman, M. Ronaghi, J.G. Mandell, K.L. Gunderson, J.H. Gundlach

Nature Biotechnology

We demonstrate that the nanopore MspA can be used to resolve the position of DNA at sub-angstrom values at bandwidths of greater than a kHz, providing the basis of a novel tool to understand enzymes.

Detection and mapping of 5-methylcytosine and 5-hydroxymethylcytosine with nanopore MspA.

A. Laszlo, I. M. Derrington, H. Brinkerhoff, K. Langford, I. Nova, J. Samson, J. Bartlett, M. Pavlenok, J. Gundlach

PNAS

Important epigenetic marker of DNA methylation is demonstrated to be easily detectable with sequencing using the nanopore MspA

Nanopore DNA sequencing with MspA.

Nanopore DNA sequencing with MspA. I.M. Derrington, M. Collins, E. Manrao, M. Pavlenok, M. Niederweis, J.H. Gundlach

PNAS

Herein we show that the Nanopore MspA has the requisite sensitivity to sequence DNA and provide a method to potentially enable sequencing with it.

Subangstrom Measurements of Enzyme Function Using a Biological Nanopore, SPRNT

Laszlo A.H., I.M. Derrington, J.H. Gundlach.

Methods Enzymol.

Using the exquisite sub-angstrom precision of the MspA nanopore we directly measure sub-catalysis reaction steps for the Hel-308 helicase elucidating a greater understanding of the mechanism of the superfamily 2 helicase.

Decoding long nanopore sequencing reads of natural DNA.

A.H. Laszlo, I. M. Derrington, B.C. Ross, H. Brinkerhoff, A. Adey, I.C. Nova, J.M. Craig, K.W. Langford, J.M. Samson, R. Daza, K. Doering, J. Shendure, J.H. Gundlach.

Nature Biotechnology

With the understood ability to sequence with the nanopore MspA, we demonstrate, for the first time multi-kb reads of genomic DNA.

Reading DNA with the MspA Nanopore and a Motor Enzyme.

Reading DNA with the MspA Nanopore and a Motor Enzyme. E. Manrao, I.M. Derrington, A. Laszlo, K. Langford, M. Hopper, N. Gilgren, M. Pavlenok, M. Niederweis, J. Gundlach.

Nat Biotechnology

We demonstrate for the first time enzyme-controlled motion of DNA through a nanopore, directly demonstrating the potential for high-throughput nanopore-sequencing.

Single Molecule DNA detection with an engineered MspA protein nanopore.

Single Molecule DNA detection with an engineered MspA protein nanopore. T. Butler, M. Pavlenok, I.M. Derrington, M. Niederweis, and J. Gundlach

PNAS

We engineer the nanopore MspA and demonstrate that such enables DNA to move through it.